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Hygromycin B solution 潮霉素溶液
 产品货号: HR2080
 产品名称: Hygromycin B solution 潮霉素溶液
 产品价格/规格: 20ml/800元
 产品说明书: 点击查看
 更新时间: 2018-02-02
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  • Hygromycin B Solution

    潮霉素B即用型溶液

    Contents20ml

    Formulation: 50 mg/ml in PBS (phosphate buffered saline), sterile filtered.

    Formula:  C20H37N3O13  CAS: 31282-04-9 MW: 527.5

    Purity: 80% (HPLC)

    Storage4

    Application:

    Hygromycin B is an aminoglycosidic antibiotic produced by Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It has been reported to interfere with translocation and to cause mistranslation. Hygromycin B is used for the selection of pro- and eukaryotic cells, stably transfected with the Hygromycin resistance gene, as well as for the maintenance of the Hygromycin phenotype of resistant cells.

    Working concentration:

    Recommended concentration for the selection of resistant cells is 50 –1000 μg/ml (see table 1). The optimal concentration is to be tested experimentally and may vary with the cell type. A commonly used concentration for selection of mammalian cells is 200μg/ml.

    Stability:

    The hygromycin B solution is stable at +2 to +8°Cfor at least five years. Some customers have been reported that frozen solution have lost complete activity[1]. Since solutions are extremely stable refrigerated, there should be no need to freeze them at-20.

    Resistance:

    A gene has been identified that confers resistance in E. coli against Hygromycin B. The resistance gene codes fore kinase that inactivates Hygromycin B through phosphorylation (Hygromycin B phosphotransferase). Cloning of the resistance gene (designated HM+, HMR, hyg or hph) and fusion with eukaryotic promoters has resulted in the construction of vectors that allow selection for resistance to Hygromycin B in both prokaryotic and eukaryotic cell systems. A variety of vectors have been developed. Hygromycin B has been used for selection of a wide variety of transfected cells (see table 1).

    Reference

    1 Perl, A. et al., Nat. Biotechnol., 14, 624-628 (1996).

    Table 1 Concentration of Hygromycin B used for selection of cells after transfection with DNA constructs encoding for Hygromycin resistance.

    Species

    Cell Type

    Cell Line/strain

    HygromycinB [μg/ml]

    E. coli

     

    JM 83

    25–100

    200

    Streptomyces lividans

     

     

    50

    Saccharomyces cerevisiae

     

     

    200

    Aspergilus nidulans

     

    GR 1

    250

    GR 5

    250

    G 191

    250

    G B 20

    750

    W 1

    >1000

    FGSC 4

    >1000

    FGSC 237

    >1000

    Tobacco

     

     

    20–200

    Chicken

     

    DT 40

    1500

    27 C 2

    1500

    RP 9

    1500

    HD 3

    1500

    CU 39

    1500

    BM 2

    1500

    Mouse

    ES cells

    LTK

    200–400

    L 929

    200

    C 127

    300

    NIH 3T3

    50–500

    PA 317

    50

    ψ2

    150

    ΩE

    50

    120

    Rat

    Embryo fibroblasts

     

    200

    Human

     

    B 95–8

    50

    Raji

    100

    721

    100

    WIL 2 TK-/-

    200

    Daudi

    200

    GG 68

    400

    293

    200

    K 562

    200

    Mouse-human

    hybrid cell

    SCC 16-5

    200

     




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