1 pI计算：https://www.protpi.ch/Calculator/ProteinTool
2 膜蛋白二级结构预测，可判断跨膜区 https://harrier.nagahama-i-bio.ac.jp/sosui/
Classification and Secondary Structure Prediction of Membrane Proteins(pdf)
3 MemBrain: Transmembrane protein structure prediction http://www.csbio.sjtu.edu.cn/bioinf/MemBrain/
上海交通大学模式识别与生物信息学研究组沈红斌教授生物信息学在线平台
4 STRING 蛋白互作 https://cn.string-db.org
5 UniProt :https://www.uniprot.org。Uniprot是 Universal Protein 的英文缩写，是信息最丰富、资源最广的蛋白质数据库。它由整合Swiss-Prot、 TrEMBL 和 PIR-PSD 三大数据库的数据而成。他的数据主要来自于基因组测序项目完成后，后续获得的蛋白质序列。
6 Cell-PLoc 2.0 www.csbio.sjtu.edu.cn/bioinf/Cell-PLoc-2/
Cell-PLoc 2.0: A package of web-servers for predicting subcellular localization of proteins in different organisms
NP文章详细解释如何操作
7 Pumba-Human https://pumba.dcsr.unil.ch/
The PUMBA database is a resource of reference 1D-SDS-PAGE migration patterns for proteins from human and other organisms. Its purpose is to validate or troubleshoot western blot experiments, but also to provide information about proteoforms.
Mylonas R, Potts A, Waridel P, Barblan J, Conde Rubio MDC, Widmann C, Quadroni M. A Database of Accurate Electrophoretic Migration Patterns for Human Proteins. J Mol Biol. 2023 Feb 28;435(4):167933. doi: 10.1016/j.jmb.2022.167933. Epub 2022 Dec 26. PMID: 36581244.