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RTU4032 GUS染色试剂盒
 产品货号: RTU4032
 产品名称: RTU4032 GUS染色试剂盒
 产品价格/规格: 50ml/500元
 产品说明书: 点击查看
 更新时间: 2020-09-26
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  • 产品介绍
  • 发表文章
  • 相关产品
  • GUS染色试剂盒(货号:RTU4032

    ●     产品组成:

    组分货号

    名称

    规格

    贮存

    运输

    RTU4032-01

    X-gluc

    粉末

    -20

    RT

    RTU4032-02

    X-gluc溶解液

    1 ml

    -20℃

    RT

    RTU4032-03

    GUS染色缓冲液

    50 ml

    4

    RT

     

    说明书

    一份

     

     

    产品简介:

    gus(β-glucuronidase,β-D- 葡萄糖苷酸酶)基因是目前常用的一种报告基因,其表达产物β-葡萄糖苷酸酶 (GUS)是一种水解酶,能催化许多β-葡萄糖苷酯类物质的水解,它可以将5-溴-4-氯-3-吲哚-β-葡萄糖苷酸酯(X-gluc.htm' target=_blank>x-gluc)分解为蓝色的物质,其检测方法简单、快速、灵敏、稳定,且背景活性低。因为绝大多数植物细胞内不存在内源的GUS活性,因此gus基因广泛用作转基因植物的报告基因,尤其是在研究外源基因瞬时表达的转化实验中广泛应用。

    该试剂盒包含GUS染色的全部试剂,使用方便,只需将配制好的X-gluc溶液和缓冲液按照比例混合即配成GUS染色液。该试剂盒可以配制50ml GUS染色液。

    贮存和效期:

    未配制的试剂盒按照温度贮存,有效期2年。

    配制好的X-gluc溶液-20℃避光保存30天,长期-80℃保存;GUS染色缓冲液 4贮存GUS染色工作液最好现用现配,短期贮存可以-20℃保存2-3

    使用说明:

    一、X-gluc溶液(50×)配制:

    吸取1ml X-gluc溶解液加入到X-gluc管中,彻底混匀,至粉末完全溶解,即配成X-gluc溶液(50×),该溶液-20℃避光保存。

    注:X-gluc溶液稳定性较差,-20℃避光保存30天左右溶液保持无色,长期保存建议按照需要分装到棕色管里,-80℃避光保存。正常的X-gluc溶液颜色为无色,如果溶液变为红色或棕色,表明溶液失效。

    .  GUS染色工作液配制:

     

    GUS染色工作液 配制量

     

    1 ml

    5 ml

    10 ml

    X-gluc溶液(50×)

    20 μl

    100 μl

    200 μl

    GUS染色缓冲液

    1 ml

    5 ml

    10 ml

    注:GUS染色工作液最好现用现配,短期贮存可以-20℃保存2-3天。

    . GUS染色步骤:

    1.取材:将叶片、花瓣、根茎等组织剪成小片,放于1.5ml离心管中。

    注:用于染色的植物材料的制备方法要因涉及的特定组织和器官的不同而异。例如,拟南芥的根、花和叶片以及烟草幼苗的根就可以不作任何预处理而直接染色。但是像烟草和马铃薯这些植物的茎和叶就必须在染色前切成薄片(1-3mm)。当操作大的组织和样品时,可以选用真空渗入法来帮助底物渗入细胞。

    2.染色:加入适量配制好的GUS染色工作液至完全覆盖材料;锡箔纸包好后摇床摇动10分钟或更长时间至材料出现蓝色。注意要做阴性和阳性对照实验。 

    3.洗脱:将材料转入无水乙醇或80%丙酮中脱色2-3次,至阴性对照材料为白色。GUS染色阳性的蓝色斑点很稳定,在酒精中不褪色。

    4.观察:一般染色后,GUS染色阳性的蓝色斑点肉眼就能看到。但是有些材料可能蓝色斑点很细微,要在显微镜下才能看到。




  • GUS染色试剂盒

    使用该产品发表部分文章列表:

    1. [IF=6.456] A PIF1/PIF3-HY5-BBX23 Transcription Factor Cascade  Affects Photomorphogenesis.

    Author: Xinyu Zhang, Fangfang Shang, Junling Huai, Gang Xu, Weijiang Tang, Yanjun Jing,Rongchen g Lin.

    Journal: Plant Physiology. 2017,Vol. 174, 2487–2500.

    Institution Institute of Botany,Chinese Academy of Sciences,Beijing,China

    Paper linkhttp://www.plantphysiol.org/content/174/4/2487

     

    2. [IF=6.17] PICKLE chromatin remodeling factorcontrols thermosensory hypocotyl growth of Arabidopsis. 

    Author: Zha P, Jing YJ, Xu, G, Lin RC

    Journal: Plant, Cell and Environment.2017,Vol40, 2426–36.

    Institution Institute of Botany,Chinese Academy of Sciences,Beijing,China

    Paper linkhttp://onlinelibrary.wiley.com/doi/10.1111/pce.13049/full

     

    3. [IF=4.3] The Wheat Bax Inhibitor-1 Protein Interacts withan Aquaporin TaPIP1 and Enhances Disease Resistance in Arabidopsis. 

    Author: Pan-Pan Lu,Tai-Fei Yu,Wei-Jun Zheng, Ming Chen, Yong-Bin Zhou,Jun Chen, You-ZhiMa, Ya-Jun Xi and Zhao-Shi Xu.

    Journal: Frontiers in Plant Science.2018,Vol.9

    InstitutionCollege of Agronomy, Northwest A&F University;

    Paper linkhttps://www.frontiersin.org/articles/10.3389/fpls.2018.00020/full

     

    4. [IF=3.33] Expression and functional analysis of two PsbS genes in bamboo (Phyllostachys edulis). 

    Author: Yongfeng Lou,Huayu Sun,Sining Wang,Hao Xu,Lichao Li,Hansheng Zhao,Zhimin Gao.

    Journal: Pysiol Plant2018

    Institution International Center for Bamboo andRattan, Beijing,China

    Paper linkhttp://onlinelibrary.wiley.com/doi/10.1111/ppl.12690/full

     

    5. [IF=3.154] MultiplePesticides Detoxification Function of Maize (Zea mays) GST34. 

    Author: Dongzhi Li, Li Xu, Sen Pang, Zhiqian Liu, Weisong Zhao, Chengju Wang.

    Journal: J. Agric. Food Chem2017 ,65 (9),pp 1847–1853

    InstitutionCollege of Science, China Agricultural University, Beijing,China

    Paper linkhttp://pubs.acs.org/doi/abs/10.1021%2Facs.jafc.7b00057

     

    6.[IF=2.87] The bambooaquaporin gene PeTIP4;1–1 confers drought and salinity tolerance in transgenicArabidopsis. 

    Author: Huayu Sun, Lichao Li, Yongfeng Lou, Hansheng Zhao, Yihong Yang, Sining Wang, Zhimin Gao.

    Journal: Plant Cell Rep  2017,Vol36, 4, 597–609

    Institution International Center for Bambooand Rattan, Beijing,China

    Paper linkhttps://link.springer.com/article/10.1007/s00299-017-2106-3

     

    7.[IF=2.236] Characterizationand Primary Functional Analysis of a Bamboo ZEP Gene from Phyllostachys edulis.

    Author: Yongfeng Lou, Huayu Sun, Lichao Li,Hansheng Zhao, Zhimin Gao.

    Journal: DNA and Cell Biology2017,Vol 36,9,1-12

    Institution International Center for Bambooand Rattan, Beijing,China

    Paper linkhttp://online.liebertpub.com/doi/abs/10.1089/dna.2017.3705

     

    8.[IF=3.687] Expression Pattern and Function Analysis of AtPPRT1, a NovelNegative Regulator in ABAand Drought Stress Responses in Arabidopsis

    Author: Linsen Pei, Lu Peng, Xia Wan, Jie Xiong, Zhibin Liu, Xufeng Li, Yi Yang andJianmei Wang.

    Journal: Int. J. Mol. Sci. 2019, 20, 394

    Institution College of Life Sciences, Sichuan University

    Paper linkhttps://www.mdpi.com/1422-0067/20/2/394

     

    9. [IF=3.93]  Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates responseto heat stress

    Author: Pan-Pan Lu, Wei-Jun Zheng, Chang-TaoWang, Wen-Yan Shi, Jin-Dong Fu, Ming Chen, Jun Chen,Yong-Bin Zhou, Ya-Jun Xi andZhao-Shi Xu.

    Journal: BMC Plant Biology 2018, 18,259

    InstitutionInstitute of Crop Science, Chinese Academy of Agricultural Sciences

    Paper link: https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-018-1485-0

     

    10. [IF=5.415]  SpHMA1 is a Chloroplast Cadmium Exporter Protecting Photochemical Reactions in the Cd Hyperaccumulator Sedum plumbizincicola

    Author: Haixia Zhao  Liangsheng Wang  Fang‐Jie Zhao  Longhua Wu Anna Liu  Wenzhong Xu.

    Journal:  Plant, Cell and Environment  12 October 2018

    Institution Institute of Botany, Chinese Academy of Sciences,Beijing,China

    Paper link: https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.13456

     

    11. [IF=8.827] SEUSS and PIF4 Coordinately Regulate Light and Temperature Signaling Pathways to Control Plant Growth.

    Author: Junling Huai, Xinyu Zhang, Jialong Li, Tingting Ma, Ping Zha, Yanjun Jing,Rongcheng Lin.

    Journal:  Molecular Plant 2018,11,928-942

    Institution Institute of Botany, Chinese Academy of Sciences,Beijing, China

    Paper link: https://doi.org/10.1016/j.molp.2018.04.005

     

    12. [IF=3.825]  AtARRE, an E3 ubiquitin ligase, negatively regulates ABA signaling in Arabidopsis thaliana.

    Author: Boya Wang,Chuzhe Li,Xiangge Kong, Ying Li, Zhibin Liu, Jianmei Wang, Xufeng Li, Yi Yang.

    Journal: Plant Cell Reports 37, 1269–1278 (2018)

    Institution College of Life Sciences, Sichuan University

    Paper link: https://doi.org/10.1007/s00299-018-2311-8

     

    13. [IF=6.902]  OsDER1 is an ER-associated protein degradation factor that responds to ER stress. Author: Dandan Qian, Guoqiang Chen, Lihong Tian, Le Qing Qu.

    Journal:  Plant Physiol 2018 178,402-412

    Institution Institute of Botany, Chinese Academy of Sciences

    Paper link: https://doi.org/10.1104/pp.18.00375

     

    14. [IF 3.93] Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates response to heat stress. Author: Pan-Pan Lu, Wei-Jun Zheng, Chang-Tao, Wang, Zhaoshi Xu.

    Journal:  BMC Plant Biology  18, 259 (2018)

    Institution Institute of Crop Science, Chinese Academy of Agricultural Sciences 

    Paper link: https://doi.org/10.1186/s12870-018-1485-0

     

    15. [IF 5.415] The chromatin-remodelling factor PICKLE interacts with CONSTANS to promote flowering in Arabidopsis.

    Author: Yanjun Jing, Qiang Guo, Ping Zha, Rongcheng Lin.

    Journal:  Plant, Cell and Environment 2019

    Institution Institute of Botany, Chinese Academy of Sciences, Beijing

    Paper link: https://doi.org/10.1111/pce.13557

     

    16. [IF 5.415] The  Arabidopsis  defensin  gene  AtPDF2.5  mediates  cadmium tolerance and accumulation.

    Author: Jin-Song Luo, Yong Yang, Tianyu Gu, Zhimin Wu, Zhenhua Zhang.

    Journal:  Plant, Cell and Environment  2019 May

    InstitutionHunan Agricultural University

    Paper link: https://doi.org/10.1111/pce.13592

     

    17. [IF 3.517] In Silico Genome-Wide Analysis of the Pear (Pyrus bretschneideri) KNOX Family and the Functional Characterization.

    Author: Xi Cheng, Yongping Cai.

    Journal: Frontiers in Genetics 2019,10 Article 632

    Institution:Anhui Agricultural University

    Paper link:  https://doi.org/10.3389/fgene.2019.00632

     

    18.[IF 3.929]  Chloroplast-Localized Protoporphyrinogen IX Oxidase1 Is Involved in the Mitotic Cell Cycle in Arabidopsis.

    Author: Jialong Li, Fan Zhang, Yuhong Li, Weicai Yang, Rongcheng Lin

    Journal: Plant and Cell Physiology 2019

    Institution Institute of Botany, Chinese Academy of Sciences, Beijing, China

    Paper link: https://doi.org/10.1093/pcp/pcz135

     

    19. [IF 3.259] Functional characterization of three MATE genes in relation to aluminum-induced citrate efflux from soybean root.

    Author: Ying Zhou, Zhengbiao Wang, Li Gong, Anle Chen, Ning Liu, Sha Li, Haoran Sun, Zhenming Yang, Jiangfeng You

    Journal: Plant Soil  443, 121–138 (2019)

    Institution:College of Plant Science, Jilin University

    Paper link: https://doi.org/10.1007/s11104-019-04192-w

     

    20. [IF 10.8] bHLH121 Functions as a Direct Link that Facilitates the Activation of FIT by bHLH IVc Transcription Factors for Maintaining Fe Homeostasis in Arabidopsis.

    Author:RihuaLei, YangLi, YuerongCai, ChenyangLi, MengnaPu, ChengkaiLu, YujieYang, GangLiang.

    Journal: Molecular Plant Vol 13, Issue 4, 6 April 2020, 634-649

    Institution:Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences

    Paper link: https://doi.org/10.1016/j.molp.2020.01.006

     

    21. [IF 11.88] A mycorrhizae-like gene regulates stem cell and gametophore development in mosses.

    Author: ShuanghuaWang, Jinling Huang

    Journal: Nature Communications 2020,11,2030

    Institution:Kunming Institute of Botany, Chinese Academy of Sciences

    Paper link: https://doi.org/10.1038/s41467-020-15967-6

     

    22. [IF 4.1] Overexpression of a Defensin-Like Gene CAL2 Enhances Cadmium Accumulation in Plants .

    Author: Jin-Song Luo,Zhenhua Zhang. 

    Journal: Frontiers in Plant Science  2020

    InstitutionHunan Agricultural University

    Paper link: https://doi.org/10.3389/fpls.2020.00217

     

    23. [IF 1.644] AtPPRT1 negatively regulates salt stress response in Arabidopsis seedlings.

    Author: Yu Liu, Linsen Pei, Shuya Xiao, Lu Peng, Zhibin Liu, Xufeng Li, Jianmei Wang.

    Journal: Plant Signaling & Behavior  2020

    InstitutionSichuan University

    Paper link: https://doi.org/10.1080/15592324.2020.1732103

     

    24. [IF 3.824] Oryza Sativa Fer-Like Fe Deficiency Induced Transcription Factor (Osfit/Osbhlh156) Interacts With Osiro2 To Regulate Iron Homeostasis.

    Author: Gang Liang, Huimin Zhang, Yang Li, Mengna Pu, Yujie Yang, Chenyang Li, Chengkai Lu,  Peng Xu, Diqiu Yu.

    Journal:  J Integr Plant Biol  2020 ,Volume 62 ,Issue 5 ,668–689

    InstitutionXishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences

    Paper link: https://doi.org/10.1111/jipb.12933

     

    25. [IF暂无] RGG1, involved in the cytokinin regulatory pathway,controls grain size in rice.

    AuthorYajun Tao, Jie Yang 

    Journal: Reaearch Square 2020

    InstitutionJiangsu Academy of Agricultural Sciences

    Paper link: https://doi.org/10.21203/rs.3.rs-40598/v1

     



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