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首页 > 常用试剂 > 即用型溶液 > 抗生素储存液

潮霉素溶液

潮霉素溶液

产品编号:HR2080

产品规格:20ml

数量
价格 ¥800

Hygromycin B Solution

潮霉素B即用型溶液

Contents20ml

Formulation: 50 mg/ml in PBS (phosphate buffered saline), sterile filtered.

Formula:  C20H37N3O13  CAS: 31282-04-9 MW: 527.5

Purity: 80% (HPLC)

Storage4

Application:

Hygromycin B is an aminoglycosidic antibiotic produced by Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It has been reported to interfere with translocation and to cause mistranslation. Hygromycin B is used for the selection of pro- and eukaryotic cells, stably transfected with the Hygromycin resistance gene, as well as for the maintenance of the Hygromycin phenotype of resistant cells.

Working concentration:

Recommended concentration for the selection of resistant cells is 50 –1000 μg/ml (see table 1). The optimal concentration is to be tested experimentally and may vary with the cell type. A commonly used concentration for selection of mammalian cells is 200μg/ml.

Stability:

The hygromycin B solution is stable at +2 to +8°Cfor at least five years. Some customers have been reported that frozen solution have lost complete activity[1]. Since solutions are extremely stable refrigerated, there should be no need to freeze them at-20.

Resistance:

A gene has been identified that confers resistance in E. coli against Hygromycin B. The resistance gene codes fore kinase that inactivates Hygromycin B through phosphorylation (Hygromycin B phosphotransferase). Cloning of the resistance gene (designated HM+, HMR, hyg or hph) and fusion with eukaryotic promoters has resulted in the construction of vectors that allow selection for resistance to Hygromycin B in both prokaryotic and eukaryotic cell systems. A variety of vectors have been developed. Hygromycin B has been used for selection of a wide variety of transfected cells (see table 1).

Reference

1 Perl, A. et al., Nat. Biotechnol., 14, 624-628 (1996).

Table 1 Concentration of Hygromycin B used for selection of cells after transfection with DNA constructs encoding for Hygromycin resistance.

Species

Cell Type

Cell Line/strain

HygromycinB [μg/ml]

E. coli

 

JM 83

25–100

200

Streptomyces lividans

 

 

50

Saccharomyces cerevisiae

 

 

200

Aspergilus nidulans

 

GR 1

250

GR 5

250

G 191

250

G B 20

750

W 1

>1000

FGSC 4

>1000

FGSC 237

>1000

Tobacco

 

 

20–200

Chicken

 

DT 40

1500

27 C 2

1500

RP 9

1500

HD 3

1500

CU 39

1500

BM 2

1500

Mouse

ES cells

LTK

200–400

L 929

200

C 127

300

NIH 3T3

50–500

PA 317

50

ψ2

150

ΩE

50

120

Rat

Embryo fibroblasts

 

200

Human

 

B 95–8

50

Raji

100

721

100

WIL 2 TK-/-

200

Daudi

200

GG 68

400

293

200

K 562

200

Mouse-human

hybrid cell

SCC 16-5

200

 




 
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