PsbC Anti-CP43 subunit of Photosystem II

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PsbC Anti-CP43 subunit of Photosystem II

产品编号：RGC2080

产品规格：20μl

数量

价格￥800

Cat No: RGC2080
PsbC | Anti-CP43 subunit of Photosystem II
Product information
Immunogen KLH-conjugated synthetic peptide chosen from known sequences of PsbC including Arabidopsis thaliana PsbC
UniProt: P56778, TAIR: AtCg00280
Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified
Format Solution Quantity 20 μl
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Application information
Recommended dilution 1:3 000-1:5000 (WB)
Expected | apparent MW 45 | 43 kDa
Confirmed reactivity Arabidopsis thaliana,Chlamydomonas reinhardtii,Chlorella sorokiniana, Chromochloris zofingiensis,Echinochloa crus-galli, Hordeum vulgare, Oryza sativa, Panax ginseng, Physcomitrella patens, Pisum sativum,Phaseolus vulgaris, Pyropia yezoensis, Synochococcus sp. PCC7002,Synechocystis sp .PCC6803, Triticum aestivu,m
Tritical, eZea mays , Verbascum lychniti, sVigna radiate

检测到两个条带，大小43和45kD

Electrophoresis: 10% RealPAGE SDS Electrophorosis(RTD6148)
Transfer: 1×RealBlot transfer buffer(RT5020)
350mA blotting to NC membrane for 30min.
Blocking: BSA at RT for 1h.
Primary antibody: PsbC 1:5000 dilution at RT for 1h
Secondary antibody: 1:5000 dilution using Goat Anti-Rabbit IgG AP
Detection: using BCIP/NBT at RT for 5 min

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