Lhca3 | PSI type III chlorophyll a/b-binding protein

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Lhca3 | PSI type III chlorophyll a/b-binding protein

产品编号：RGL2080

产品规格：20μl

数量

价格￥800

Product no RGL2080
Lhca3 | PSI type III chlorophyll a/b-binding protein
Product information
Immunogen BSA-conjugated synthetic peptide derived from the Lhca3 protein sequence from Arabidopsis thaliana UniProt:
Q9SY97, TAIR: At1g61520. This sequence is highly conserved in Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms.
Host Rabbit Clonality Polyclonal
Purity Total IgG
Format Solution
Quantity 20 μl
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Application information
Recommended dilution 1 : 2000-1 : 5000 (WB)
Expected | apparent MW 29 | 25 kDa for Arabidopsis thaliana (due to a transit peptide being cleaved off)
Confirmed reactivity Arabidopsis thaliana, Aracis hypogae, Bryopsis corticulans, Citrus reticulata, Hordeum vulgare, Oryza sativa,
Pisum sativum, Phaseolus vulgaris, Posidonia oceanica, Spinacia oleracea, Triticum aestivum, Triticale, Zea mays
Predicted reactivity Dicots, Gymnosperms
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Application Example
检测到条带，25 kD

检测到条带，25 kD
Electrophoresis: 12% RealPAGE SDS Electrophorosis(RTD6148)
Transfer: 1×RealBlot transfer buffer(RT5020)
350mA blotting to NC membrane for 30min.
Blocking: BSA at RT for 1h.
Primary antibody: Lhca3 1:5000 dilution at RT for 1h
Secondary antibody: 1:5000 dilution using Goat Anti-Rabbit IgG AP
Detection: using BCIP/NBT at RT for 5 min

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