Zeocin Selection Reagent

● 产品规格

Zeocin粉末型

● 产品说明

Zeocin? Reagent is a member of the bleomycin antibiotic family. Resistance is conferred by the Sh ble gene which encodes a 13,665 dalton protein. In cells expressing the protein, Zeocin? is prevented from binding and cleaving the cellular DNA. Zeocin? is effective on most aerobic cells and can be used for selection in mammalian and insect cell lines, yeast, and bacteria. The amount of Zeocin? required for selection will vary between 50-2000 μg/ml (typically 300μg /ml) depending on the cell type.

Zeocin可选择Sh ble基因表达的细胞。与现在使用的其他动物细胞标记物无交叉抗性。因此这种抗生素可用来分离对其他筛选剂（如：庆大霉素，潮霉素）有抗性的克隆。Zeocin是一种属于争光霉素家族的糖蛋白抗生素，在体内能作用于大多数细菌（包括E. coli）、真菌（如：酵母菌）、植物细胞、动物细胞。

● 液体Zeocin可用于细胞培养。

● 用于筛选转化株的Zeocin浓度根据pH值和盐浓度改变。pH越高，盐浓度越低，Zeocin活性越大。

● 普通大肠杆菌菌株筛选可在25 μg/ml zeocin浓度的低盐LB琼脂培养基（yeast extract 5 g/L, Tryptone 10 g/L, NaCl 5 g/L, Agar 15 g/L,pH 7.5）中进行，pH调至7.5 。

● 动物细胞筛选：根据不同细胞系从100 到 750 μg/ml。

● 植物细胞筛选：根据不同的细胞种类和培养基从50 到 250 μg/ml 。

● 酵母筛选：取决于种属和培养基，S. cerevisiae Sh ble转化株在50 μg/ml 浓度的YPD培养基中筛选，pH调至7.0。

● 粉末型配制方法：

取1 ml Zeocin溶解液加到Zeocin干粉中，彻底溶解，用0.22 μm 一次性滤器抽滤除菌，即配成1 ml（100mg/ml）的Zeocin溶液，-20℃保存。

● 技术参数：

Empirical formula: C₅₅H₈₅O₂₁N₂₀S₂Cu·HCl

Molecular weight: 1525

CAS：11006-33-0

Zeocin

使用该产品发表部分文章列表：

1. [2022 IF=11.4] Random mutagenesis and transcriptomics-guided rational engineering in Zygosaccharomyces rouxii for elevating D-arabitol biosynthesis.

Author: Guoyan Zhang, Hossain M Zabed, Yufei Zhang, Jia Li, Junhua Yun, Xianghui Qi

Journal: Bioresource Technology 400 (2024) 130685

Institution： School of Life Sciences, Guangzhou University

Paper link：https://doi.org/10.1016/j.biortech.2024.130685