5M甜菜碱溶液-PCR级
5MBetaine Solution-PCR级
● 产品货号及规格：
|
货号
|
名称
|
规格
|
贮存
|
|
RTE3103-02
|
5M 甜菜碱溶液
|
5×1 ml
|
-20℃
|
● 产品简介：
Betaine, DMSO and formamide helps in reducing the secondary
structure of GC rich templates and assists amplification of these templates.
Betaine is used at3.5Mto0.1M, DMSO should be used between 2-8%,
however 10% DMSO can reduce Taq polymerase activity by up to 50%. Formamide is
generally used at 1-5%. It has been our experience that the use of DMSO in
combination with betaine is superior to using formamide.
● 贮存：-20℃贮存。有效期一年。
● 使用说明：
1．-20℃保存的甜菜碱溶液常温融化，混匀。
2. 根据根据PCR反应体系，加入适量体积的5M甜菜碱溶液。
|
|
20 μl 体系
|
反应体系终浓度
|
|
10×PCR Buffer
|
2 μl
|
1×
|
|
5M Betaine*
|
4 μl
|
1 M
|
|
模板
|
0.05-0.5μg
|
as you wish
|
|
上游引物 10μM
|
0.5 μl
|
0.2 μM
|
|
下游引物 10μM
|
0.5 μl
|
0.2 μM
|
|
dNTP10mM
|
0.5 μl
|
200 μM
|
|
Taq
|
0.5-1 μl
|
2.5-5 U
|
|
灭菌水
|
up to 20 μl
|
|
注*：不同模板，不同片段的扩增可能需要不同浓度的甜菜碱，可以适当调节加入体积，找到适合自己反应的甜菜碱浓度。
● References：
1 Rees, William A., et al., Betaine can eliminate the
base pair composition dependence of DNA melting. Biochemistry, 32, 137-144 (1993)