DNA Marker I plus（100-700 bp）

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DNA Marker I plus（100-700 bp）

产品编号：RTM436-02

产品规格：100次

数量

价格￥150

精准定量 DNA Marker I plus

● 产品编号及规格：

RTM436-02 100T(2×250μl)

本产品是由7条带状双链DNA条带组成，适用于琼脂糖凝胶电泳中DNA条带的分析。产品中已含有1×loading buffer，可根据实验需要，直接取2-5 μl电泳，使用方便，电泳图像清晰。7条带的大小和含量分别为100（50 ng/5 μl），200（50 ng/μl），300(50 ng/μl)，400（100 ng/μl），500（50 ng/μl），600(50 ng/μl)，700（50 ng/μl）bp。其中400bp条带加亮显示。

● 储存及运输：

4℃贮存3个月， -20℃长期贮存；常温运输。

● 使用方法：

1. 取2-5 μl本产品加入到琼脂糖凝胶的加样孔中就行电泳。

注：根据电泳梳子的厚度和宽度确定上样量。每1mm×1mm（厚度×宽度）加样孔上样1μl；窄齿梳子（一般为1mm×2mm）上样2μl；宽齿梳子（一般为1mm×5mm）上样5μl。如果使用厚齿梳子或宽于5mm的梳子，可以适当调整上样量。

2. 建议电泳条件：凝胶浓度为2.0％，凝胶长度5-7 cm，电泳电压~7 v/cm，电泳时间30-35分钟。

3. 通过核酸染料染色后紫外灯或蓝光仪下观察条带。

● 注意事项：

1. 琼脂糖的质量对DNA的电泳有很大影响，电泳时请尽量使用质量优等的琼脂糖。由于PAGE电泳的特殊性，该产品不建议用于PAGE电泳。

2. 使用与电泳缓冲液一致的缓冲液融化琼脂糖，例如使用1×TAE缓冲液电泳，需使用1×TAE缓冲液溶胶。

3. 多次电泳后缓冲液电导增强，相同电压下电流增大，导致分辨率降低，并且明显产热，严重时会引起凝胶熔化或DNA变性。

4. 推荐使用RealSafe系列染料（货号：GR002，GR003，GR004）染色。如果使用Goldview或EB等染料进行染色，由于此类染料带更多的正电荷（染料移动方向与核酸泳动方向相反），随着电泳时间延长，染料向大片段聚集，凝胶会出现阴阳胶现象（染料含量高的凝胶紫外下较亮，含量低的凝胶紫外下发暗），导致小分子量DNA显色很弱或不可见。阴阳胶可以在电泳结束后浸泡染色，将胶浸没在含1 μg /ml EB或Goldview的电泳缓冲液中20-25 min，小分子量DNA会显色清楚。更长时间浸泡会因DNA分子扩散而使条带弥散，小分子量DNA更易弥散。

5. 紫外照射下EB易分解，使DNA条带荧光变浅。DNA受紫外光照射形成嘧啶二聚体，不利于待回收片段的下游工作，因此尽可能减少紫外照射时间。

6. 蔗糖会结合DNA，降低其电泳迁移率，建议DNA样品上样使用含甘油的Loading Buffer。

7. 大部分核酸工具酶会结合DNA，降低其电泳迁移率，大分子量DNA尤为明显。

● 实验示例：

● 附录：

DNA在琼脂糖凝胶中的有效分离范围

琼脂糖浓度%（w/v）	双链DNA有效分离范围（bp）	优选电泳缓冲液	示踪染料相当于双链DNA片段粗略大小(bp)
			溴酚蓝		二甲苯菁
			TBE	TAE	TBE	TAE
0.5	2000-50000	1×TAE	750	1150	13000	16700
0.8	800-10000	1×TAE	320	530	4830	6500
1.0	400-8000	1×TAE	220	370	3030	4160
1.2	300-7000	1×TAE/0.5×TBE	160	275	2070	2890
1.5	200-3000	1×TAE/0.5×TBE	110	190	1300	1840
2.0	100-2000	0.5×TBE	65	120	710	1040
3.0	25-1000	0.5×TBE	30	60	300	460

使用DNA Marker产品发表部分文章列表：

1. [2021 IF=1.17] Dendrostoma covidicola sp. nov. (Erythrogloeaceae, Diaporthales) on Fagus sylvatica from Sichuan Province, China.

Author: MILAN C. SAMARAKOON, ITTHAYAKORN PROMPUTTHA & JIAN-KUI (JACK) LI

Marker: RTM415，D2000 DNA ladder

Journal: Phytotaxa. 2021，483 (2): 085–094.

Institution：University of Electronic Science and Technology of China

Paper link：https://doi.org/10.11646/phytotaxa.483.2.1

2.[2021 IF=6.057] MUC1 detection and in situ imaging method based on aptamer conformational switch and hybridization chain reaction.

Marker :RTM403，DNA Marker III

Author: ZihanSong，ShengCai.

Journal: Talanta. Vol 239, 2022, 123129

Institution： Zhejiang University

Paper link：https://doi.org/10.1016/j.ijbiomac.2021.11.194

3. [2021 IF=3.24] Curing of Plasmid pXO1 from Bacillus anthracis Using Plasmid Incompatibility.

Marker :RTM404，DNA Marker IV

Author: Xiankai Liu, Dongshu Wang, Huagui Wang, Erling Feng, Li Zhu, Hengliang Wang

Journal: PLos one，January 2012 | Volume 7 | Issue 1 | e29875

Institution：State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology

Paper link： https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0029875

4. [2021 IF=3.24] Identification of a Novel Fungus, Leptosphaerulina chartarum SJTU59 and Characterization of Its Xylanolytic Enzymes.

Marker :RTM409，100bp DNA ladder

Author: Qiong Wu, Yaqian Li, Yingying Li, Shigang Gao, Meng Wang, Tailong Zhang, Jie Chen

Journal: PLos one，September 2013 | Volume 8 | Issue 9 | e737

Institution：School of Agriculture and Biology, Shanghai Jiao Tong University

Paper link：https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0073729

5. [2021 IF=3.08] Low genetic diversity and local adaptive divergence of Dracaena cambodiana (Liliaceae) populations associated with historical population bottlenecks and natural selection: an endangered long-lived tree endemic to Hainan Island, China.

Marker :RTM409，100bp DNA ladder

Author: D.-J. Zheng, L.-S. Xie, J.-H. Zhu，Z.-L. Zhang

Journal: Plant Biology，14 (2012) 828–838

Institution：Key Laboratory of Crop Genetics and Breeding of Hainan Province, Hainan Academy of Agricultural Sciences

Paper link：https://onlinelibrary.wiley.com/doi/10.1111/j.1438-8677.2011.00559.x

6. [2021 IF=3.24] Envelope Proteins ofWhite Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT).

Marker :RTM415，D2000 DNA ladder

Author: Shijun Xie, Xiaojun Zhang, Jiquan Zhang, Fuhua Li, Jianhai Xiang

Journal: Plos One，Published: December 21, 2015

Institution：Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences

Paper link： https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144922

7. [2021 IF=1.17] Rhopalostroma brevistipitatum sp. nov. from Thailand with an extended generic description for Rhopalostroma.

Marker :RTM415，D2000 DNA ladder

Author: DINUSHANI A. DARANAGAMA, XINGZHONG LIU, SUNITA CHAMYUANG, MARC STADLER，ALI H.BAHKALI, KEVIN D. HYDE

Journal: Phytotaxa，2015，227 (3): 229–242

Institution：State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link：https://www.biotaxa.org/Phytotaxa/article/view/phytotaxa.227.3.2/0

8. [2021 IF=20.37] A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis)

Marker :RTM415，D2000 DNA ladder

Author: Dhanushka Udayanga & Xingzhong Liu &Pedro W. Crous & Eric H. C. McKenzie &Ekachai Chukeatirote & Kevin D. Hyde

Journal: Fungal Diversity (2012) 56:157–171

Institution：State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link：https://link.springer.com/article/10.1007/s13225-012-0190-9

9. [2021 IF=2.9] An easily-performed high-throughput method for plant genomic DNA extraction.

Marker :RTM421，λ DNA/HindIII

Author: Deshui Yu, Ju Zhang, Guangxuan Tan, Ningshu Yu, Qiuyue Wang, Qiqi Duan, Xin

Qi, Mingjiao Cheng, Chunxue Yan, Zhangkun Wei, Zhenmiao Yu, Wenchao Huang, Chengwei Li

Journal: Analytical Biochemistry Volume 569, 15 March 2019, Pages 28-30

Institution：Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal

University

Paper link：

https://www.sciencedirect.com/science/article/abs/pii/S0003269718310637?via%3Dihub

10. [2021 IF=2.88] Genetic diversity and genetic structure of Decalobanthus boisianus in Hainan Island, China.

Marker :RTM415，D2000 DNA ladder

Author: Huan Jiang，Wenxing Long， Hui Zhang，Chengneng Mi，Tao Zhou，Zongzhu Chen

Journal: Ecology and Evolotion Volume 569, 15 March 2019, Pages 28-30

Institution：Institute of Tropical Agriculture and Forestry, Hainan University

Paper link： https://doi.org/10.1002/ece3.5127

11. [2021 IF=5.64] Identi?cation of a Novel Hypovirulence-Inducing Hypovirus From Alternaria alternata.

Marker :RTM406，DNA Marker VI

Author: Huan Li, Ruiling Bian, Qian Liu, Liu Yang, Tianxing Pang, Lakha Salaipeth,

Ida Bagus Andika, Hideki Kondo and Liying Sun

Journal: Frontiers in Microbiology May 2019 | Volume 10 | Article 1076

Institution：State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University

Paper link： https://www.frontiersin.org/articles/10.3389/fmicb.2019.01076/full

12. [2021 IF=2.6] Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol.

Marker :RTM415，D2000 DNA ladder

Author: Dongmei Jiang, Dizhe Wei, Hongtao Li, Liuqing Wang, Nan Jiang,Yunchao Li and Meng Wang

Journal: Journal of the Science of Food and Agriculture Volume101, Issue7，2021，3071-3077

Institution：Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences

Paper link：https://onlinelibrary.wiley.com/doi/10.1002/jsfa.10901

13. [2021 IF=20.37] Taxonomy, Phylogeny, Molecular Dating and Ancestral State Reconstruction of Xylariomycetidae (Sordariomycetes)

Marker :RTM415，D2000 DNA ladder

Author: Kevin D Hyde，Jian-Kui Liu

Journal: Fungal Diversity volume 112, pages 1–88 (2022)

Institution：School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China

Paper link：https://link.springer.com/article/10.1007/s13225-021-00495-5

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