精准定量D500 DNA ladder

 ● 产品编号及规格：

    RTM439-02       100 T(2×250 μl)

本产品是由9条精准定量的带状双链DNA条带组成，适用于琼脂糖凝胶电泳中DNA条带的定量分析。产品中已含有1×loading buffer，可根据实验需要，直接取2-5 μl电泳，使用方便，电泳图像清晰。如取5 μl上样，9条带的大小和含量分别为50 bp（50 ng），100 bp（50 ng），150 bp（50 ng），200 bp（50 ng），250 bp（100 ng），300 bp（50 ng），350 bp（50 ng），400 bp（50 ng），500 bp（50 ng），其中250 bp条带加亮显示。

● 储存及运输：

  4℃贮存3个月， -20℃长期贮存；常温运输。

● 使用方法：

1. 取2-5 μl本产品加入到琼脂糖凝胶的加样孔中（每1mm×1mm加样孔加1μl， 

如果加样孔宽于5mm，可以适当增加上样量）进行电泳。

2. 建议电泳条件：凝胶浓度为2.0％，凝胶长度5-7cm，电泳电压~7 v/cm，电泳时间30-35分钟。

3. 通过核酸染料染色后紫外灯或蓝光仪下观察条带。

● 注意事项：

1. 琼脂糖的质量对DNA的电泳有很大影响，电泳时请尽量使用质量优等的琼脂糖。由于PAGE电泳的特殊性，该产品不建议用于PAGE电泳。

2. 使用与电泳缓冲液一致的缓冲液融化琼脂糖，例如使用1×TAE缓冲液电泳，需使用1×TAE缓冲液溶胶。

3. 多次电泳后缓冲液电导增强，相同电压下电流增大，导致分辨率降低，并且明显产热，严重时会引起凝胶熔化或DNA变性。

4. 推荐使用RealSafe系列染料（货号：GR002，GR003，GR004）染色。如果使用Goldview或EB等染料进行染色，由于此类染料带更多的正电荷（染料移动方向与核酸泳动方向相反），随着电泳时间延长，染料向大片段聚集，凝胶会出现阴阳胶现象（染料含量高的凝胶紫外下较亮，含量低的凝胶紫外下发暗），导致小分子量DNA显色很弱或不可见。阴阳胶可以在电泳结束后浸泡染色，将胶浸没在含1 μg /ml EB或Goldview的电泳缓冲液中20-25 min，小分子量DNA会显色清楚。更长时间浸泡会因DNA分子扩散而使条带弥散，小分子量DNA更易弥散。

5. 紫外照射下EB易分解，使DNA条带荧光变浅。DNA受紫外光照射形成嘧啶二聚体，不利于待回收片段的下游工作，因此尽可能减少紫外照射时间。

6. 蔗糖会结合DNA，降低其电泳迁移率，建议DNA样品上样使用含甘油的Loading Buffer。

7. 大部分核酸工具酶会结合DNA，降低其电泳迁移率，大分子量DNA尤为明显。

● 实验示例：

● 附录： 

DNA在琼脂糖凝胶中的有效分离范围

使用DNA Marker产品发表部分文章列表：

1. [2021 IF=1.17] Dendrostoma covidicola sp. nov. (Erythrogloeaceae, Diaporthales) on Fagus sylvatica from Sichuan Province, China.

Author: MILAN C. SAMARAKOON, ITTHAYAKORN PROMPUTTHA & JIAN-KUI (JACK) LI

Marker: RTM415，D2000 DNA ladder

Journal: Phytotaxa. 2021，483 (2): 085–094.

Institution：University of Electronic Science and Technology of China

Paper link：https://doi.org/10.11646/phytotaxa.483.2.1

2.[2021 IF=6.057] MUC1 detection and in situ imaging method based on aptamer conformational switch and hybridization chain reaction.

Marker :RTM403，DNA Marker III

Author: ZihanSong，ShengCai.

Journal: Talanta. Vol 239, 2022, 123129

Institution： Zhejiang University

Paper link：https://doi.org/10.1016/j.ijbiomac.2021.11.194

3. [2021 IF=3.24] Curing of Plasmid pXO1 from Bacillus anthracis Using Plasmid Incompatibility.

Marker :RTM404，DNA Marker IV

Author: Xiankai Liu, Dongshu Wang, Huagui Wang, Erling Feng, Li Zhu, Hengliang Wang

Journal: PLos one，January 2012 | Volume 7 | Issue 1 | e29875

Institution：State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology

Paper link： https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0029875

4. [2021 IF=3.24] Identification of a Novel Fungus, Leptosphaerulina chartarum SJTU59 and Characterization of Its Xylanolytic Enzymes.

Marker :RTM409，100bp DNA ladder

Author: Qiong Wu, Yaqian Li, Yingying Li, Shigang Gao, Meng Wang, Tailong Zhang, Jie Chen

Journal: PLos one，September 2013 | Volume 8 | Issue 9 | e737

Institution：School of Agriculture and Biology, Shanghai Jiao Tong University

Paper link：https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0073729

5. [2021 IF=3.08] Low genetic diversity and local adaptive divergence of Dracaena cambodiana (Liliaceae) populations associated with historical population bottlenecks and natural selection: an endangered long-lived tree endemic to Hainan Island, China.

Marker :RTM409，100bp DNA ladder

Author: D.-J. Zheng, L.-S. Xie, J.-H. Zhu，Z.-L. Zhang

Journal: Plant Biology，14 (2012) 828–838

Institution：Key Laboratory of Crop Genetics and Breeding of Hainan Province, Hainan Academy of Agricultural Sciences

Paper link：https://onlinelibrary.wiley.com/doi/10.1111/j.1438-8677.2011.00559.x

6. [2021 IF=3.24] Envelope Proteins ofWhite Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT).

Marker :RTM415，D2000 DNA ladder

Author: Shijun Xie, Xiaojun Zhang, Jiquan Zhang, Fuhua Li, Jianhai Xiang

Journal: Plos One，Published: December 21, 2015

Institution：Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences

Paper link： https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144922

7. [2021 IF=1.17] Rhopalostroma brevistipitatum sp. nov. from Thailand with an extended generic description for Rhopalostroma.

Marker :RTM415，D2000 DNA ladder

Author: DINUSHANI A. DARANAGAMA, XINGZHONG LIU, SUNITA CHAMYUANG, MARC STADLER，ALI H.BAHKALI, KEVIN D. HYDE

Journal: Phytotaxa，2015，227 (3): 229–242

Institution：State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link：https://www.biotaxa.org/Phytotaxa/article/view/phytotaxa.227.3.2/0

8. [2021 IF=20.37] A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis)

Marker :RTM415，D2000 DNA ladder

Author: Dhanushka Udayanga & Xingzhong Liu &Pedro W. Crous & Eric H. C. McKenzie &Ekachai Chukeatirote & Kevin D. Hyde

Journal: Fungal Diversity (2012) 56:157–171

Institution：State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link：https://link.springer.com/article/10.1007/s13225-012-0190-9

9. [2021 IF=2.9] An easily-performed high-throughput method for plant genomic DNA extraction.

Marker :RTM421，λ DNA/HindIII

Author: Deshui Yu, Ju Zhang, Guangxuan Tan, Ningshu Yu, Qiuyue Wang, Qiqi Duan, Xin

Qi, Mingjiao Cheng, Chunxue Yan, Zhangkun Wei, Zhenmiao Yu, Wenchao Huang, Chengwei Li

Journal: Analytical Biochemistry Volume 569, 15 March 2019, Pages 28-30

Institution：Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal

University

Paper link：

https://www.sciencedirect.com/science/article/abs/pii/S0003269718310637?via%3Dihub

10. [2021 IF=2.88] Genetic diversity and genetic structure of Decalobanthus boisianus in Hainan Island, China.

Marker :RTM415，D2000 DNA ladder

Author: Huan Jiang，Wenxing Long， Hui Zhang，Chengneng Mi，Tao Zhou，Zongzhu Chen

Journal: Ecology and Evolotion Volume 569, 15 March 2019, Pages 28-30

Institution：Institute of Tropical Agriculture and Forestry, Hainan University

Paper link： https://doi.org/10.1002/ece3.5127

11. [2021 IF=5.64] Identi?cation of a Novel Hypovirulence-Inducing Hypovirus From Alternaria alternata.

Marker :RTM406，DNA Marker VI

Author: Huan Li, Ruiling Bian, Qian Liu, Liu Yang, Tianxing Pang, Lakha Salaipeth,

Ida Bagus Andika, Hideki Kondo and Liying Sun

Journal: Frontiers in Microbiology May 2019 | Volume 10 | Article 1076

Institution：State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University

Paper link： https://www.frontiersin.org/articles/10.3389/fmicb.2019.01076/full

12. [2021 IF=2.6] Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol.

Marker :RTM415，D2000 DNA ladder

Author: Dongmei Jiang, Dizhe Wei, Hongtao Li, Liuqing Wang, Nan Jiang,Yunchao Li and Meng Wang

Journal: Journal of the Science of Food and Agriculture Volume101, Issue7，2021，3071-3077

Institution：Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences

Paper link：https://onlinelibrary.wiley.com/doi/10.1002/jsfa.10901

13. [2021 IF=20.37] Taxonomy, Phylogeny, Molecular Dating and Ancestral State Reconstruction of Xylariomycetidae (Sordariomycetes)

Marker :RTM415，D2000 DNA ladder

Author: Kevin D Hyde，Jian-Kui Liu

Journal: Fungal Diversity volume 112, pages 1–88 (2022)

Institution：School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China

Paper link：https://link.springer.com/article/10.1007/s13225-021-00495-5

14. [2021 IF=8.025] A method for high-concentration agarose gel preparation and its

application in high-resolution separation of low-molecular-weight nucleic acids and proteins.

Marker :RTM443，10bp DNA ladder

Author: Lili Chang , Dan Wang, Cunzhi Peng, Qi Wang, Bingqiang Xu, Zheng Tong

Journal: International Journal of Biological Macromolecules 231 (2023) 123358

Institution：Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural

Sciences

Paper link：https://doi.org/10.1016/j.ijbiomac.2023.123358