Timentin溶液(100mg/ml)
Timentin Solution(100mg/ml)
● 产品包装:
产品编号
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产品名称
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产品包装
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说明书
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TR4201S
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Timentin溶液(100 mg/ml)
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10×1ml
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1份
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CAS: 4697-14-7/61177-45-5(替卡西林钠/克拉维酸钾)
Formula: C15H14N2Na2O6S2/C8H8KNO5
Appearance: pale
yellow clear Solution
Typical Working
Concentration: 50-200 mg/L
● 产品简介:
Timentin is a suitable antibiotic for
Agrobacterium counterselection and a mixture of two antibiotics: ticarcillin
and clavulanic acid. Most wild Agrobacterium isolates have beta-lactamase
activity which clavulanic acid exhibits activity towards. Many Agrobacterium
isolates are highly susceptible to Timentin. Agrobacterium strains carrying the
standard pBR beta-lactamase gene are also Timentin (clavulanic acid)
susceptible. Timentin killing of Agrobacterium wild-type strains is three logs
greater than with comparable doses of carbenicillin.
In plant transformation experiments, 100
μg/ml Timentin is sufficient to counterselect Agrobacterium. To improve
efficacy (of any antimicrobial), solid media are best slightly dried before
use. At these concentrations, Timentin exhibits no phytotoxicity to Arabidopsis
root cultures.
● 保存条件:
-20℃保存。有效期12个月。
● References:
1
Zimmerman TW (1995) Effect of Timentin for controlling Agrobacterium
tumefaciens following cocultivation on select plant species. In Vitro Cell Dev Biol 31:70A
2 Cheng Z M et al. Timentin as an alternative
antibiotic for suppression of Agrobacterium tumefaciens in genetic
transformation. Plant Cell Reports (1998)
17: 646–649