免费咨询电话: 400-699-0631      点击这里给我发消息
 
 
 
产品分类
 
·PCR
 
 
 





RTD6101 超低分子量蛋白Marker I(3.3-22KD)
 产品货号: RTD6101
 产品名称: RTD6101 超低分子量蛋白Marker I(3.3-22KD)
 产品价格/规格: 10次(100μl) 200元
 产品说明书: 点击查看
 更新时间: 2022-08-29
详细信息   访问次数:

  • 产品介绍
  • 发表文章
  • 相关产品
  • 质检证书
  • 超低分子量蛋白质Marker I3.3-22kD

    Ultra Low Molecular Weight Protein Marker I

    产品编号及规格:

    RTD6101   10T100μl)   200元

     储存条件:

       -20保存,开封后有效期12个月。

     产品简介:

    本产品包含3种多肽和2种低分子量蛋白质组成,分子量范围为3.3kD-22kD。可以用来判断 SDS-PAGE上多肽和小蛋白的分子量。


    ● 使用说明:

    第一次收到该产品,常温融化后,彻底混匀,离心快甩将溶液完全收集到管底

    . 制胶:

    I 配制分离胶

    1.按照表一将不同体积的双蒸水、40% PAA19:1)、4×凝胶缓冲液(货号:GB010)和乙二醇加入到小烧杯中混合。

    2.加入10%APSTEMED,立即混匀5-10秒,以使溶液充分混匀。

    3.在凝胶模具中迅速灌入适量分离胶溶液,然后在分离胶溶液上轻轻覆盖一层1 cm的无水乙醇,使凝胶表面保持平整。

    4.静置15-30分钟,待分离胶和乙醇层之间出现一个清晰的界面表示凝胶已聚合。

    表一  (一块1 mm mini胶用量)

     

    分离胶

    浓缩胶

     

    18T, 5%C /5 ml

    5T, 3.3%C/2 ml

    40% PAA19:1

    2.25 ml

    /

    40% PAA (29:1)

    /

    0.25 ml

    4×凝胶缓冲液

    1.25 ml

    0.5 ml

    乙二醇(电泳级)

    1.5 ml

    /

    ddH2O

    /

    1.25ml

    10APS

    50 μl

    20 μl

    TEMED

    5 μl

    2 μl


    II 配制浓缩胶

    去除覆盖在分离胶上的乙醇层,用滤纸将残留乙醇吸去。

    1.按照表一将不同体积的双蒸水、40%PAA(29:1)和4×凝胶缓冲液加入到小烧杯中混合。

    2.加入10%过硫酸铵和TEMED,立即混匀5-10秒,以使溶液充分混匀。

    3.将梳子插入凝胶内,避免产生气泡。

    4.静置30-60分钟装待凝胶聚合

    . 电泳:

    1. Marker样品,充分混匀后备用。不要加热处理。

    2. 电泳前,将10×阳极缓冲液(Cat No:AB080)和10×阴极缓冲液(Cat No:CB010)用蒸馏水稀释成1×缓冲液备用。将电泳槽的外槽加入1×阳极缓冲液,内槽加入1×阴极缓冲液,轻柔拔出梳子,将Marker或蛋白样品(已经过2×Tricine上样缓冲液[Cat No:TP050]处理)加入点样孔,参考下表进行电泳,至每条带都清晰可见时停止电泳。整个电泳过程大约需要2-3个小时。

    表二 多肽电泳条件

    恒电压

    150V

    起始电流

    60-75mA/板胶

    结束电流

    15-25mA/板胶

    电泳时间

    2.5-3小时



    多肽电泳配套试剂

    名称

    货号

    规格

    超低分子量蛋白电泳试剂盒

    RTD6120

    10

    超低分子量蛋白Marker I3.3-20.1 kD

    RTD6101

    10次(100 μl

    超低分子量蛋白Marker III3.4-100 kD

    RTD6125

    10次(50 μl

    彩虹预染超低分子量蛋白Marker1.2-45 kD

    RTD6110

    10 50 μl

    彩虹预染超低分子量蛋白Marker II3-40 kD

    RTD6145

    10 50 μl

    50次(250 μl

    40% PAA19:1

    AC1914-01

    100ml

    40% PAA29:1

    AC2914-01

    100ml

    多肽电泳凝胶缓冲液

    GB010

    100ml

    10×Tris-Tricine-SDS电泳缓冲液(干粉型)

    CB010P

    500ml

    2×Tricine 上样缓冲液

    TP050

    10×1ml

    FastBlue蛋白染色液

    RTD6202

    500ml

    乙二醇(电泳级)

    EA0582-02

    100 ml

    10×阳极缓冲液 (多肽电泳用

    AB080

    250 ml

    10×阴极缓冲液(多肽电泳用)

    CB010

    100 ml



  • 使用蛋白Marker产品发表部分文章列表:

    1. [2021 IF=5.5] Garviecin LG34, a novel bacteriocin produced by Lactococcus garvieaeisolated from traditional Chinese fermented cucumber.

    Author: Yurong Gao, Dapeng Li, Shan Liu, Liyuan Zhang

    Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

    Journal: Food Control 50 (2015) 896e900

    Institution:Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

    Paper linkhttp://dx.doi.org/10.1016/j.foodcont.2014.10.040

     

    2. [2021 IF=5.5] Purification and partial characterization of M1-UVs300, a novel bacteriocin produced by   isolated from fermented sausage Lactobacillus plantarum.

    Author: Yu An, Ying Wang, Xiaoyue Liang, Huaxi Yi, Zhaohang Zuo, Xiaoxi Xu, Dongjie

    Zhang, Changqing Yu, Xue Han

    Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

    Journal: Food Control  Volume 81, November 2017, Pages 211-217

    Institution:Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

    Paper linkhttps://doi.org/10.1016/j.foodcont.2017.05.030

     

    3. [2021 IF=5.75] The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System.

    Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Jingjing Yuan and Dewen Qiu

    Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

    Journal: Frontiers in Plant Science.  Volume 81, November 2017, Pages 211-217

    InstitutionInstitute of Plant Protection, Chinese Academy of Agricultural Sciences

    Paper linkhttps://doi.org/10.1093/jxb/ery351

     

    4. [2021 IF=2.99] Purifcation, characterization, and action mechanism of plantaricin DL3, a novel bacteriocin against Pseudomonas aeruginosa produced by Lactobacillus plantarum DL3 from Chinese Suan Tsai.

    Author: Xinran Lv,  Yang Lin ,Yu Jie, Mengtong Sun, Bolin Zhang,Fengling Bai,Hongfei Zhao,Jianrong Li

    Marker: RTD6127,超低分子量蛋白Marker IV(3.3-45 kD)

    Journal: European Food Research and Technology. Vol 244, pages 323–331 (2018)

    InstitutionCollege of Biological Science and Biotechnology, Beijing Forestry University

    Paper linkhttps://link.springer.com/article/10.1007/s00217-017-2958-3

     

    5. [2021 IF=6.99] Verticillium dahliae Alt a 1-like protein PevD1 targets cotton PR5-like protein and contributes to fungal infection.

    Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Dewen Qiu

    Marker: RTD6110,彩虹超低分子量蛋白Marker (1.2-45 kD)

    Journal: Journal of Experimental Botany, Vol 70, Issue 2, 15 January 2019,613-626

    InstitutionInstitute of Plant Protection, Chinese Academy of Agricultural Sciences

    Paper linkhttps://doi.org/10.1093/jxb/ery351

     

    6. [2021 IF=11.277] An  Alternative  Splicing  Variant  of  PtRD26  Delays  Leaf  Senescence by Regulating Multiple NAC Transcription Factors in Populus.

    Author: Hou-Ling Wang,Yi Zhang,Ting Wang, Qi Yang,Yanli Yang,Ze Li, Bosheng Li, Xing Wen, Wenyang Li, Weilun Yin, Xinli Xia, Hongwei Guo, and Zhonghai Li

    Marker: RTD6110,彩虹预染超低分子量蛋白(1.2-45 kD)

    Journal: Plant Cell. Volume 33, Issue 5, May 2021, Pages 1594–1614

    InstitutionBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University

    Paper linkhttps://doi.org/10.1093/plcell/koab046

     

    7. [2021 IF=2.89] Rapid and sensitive detection ofStaphylococcus aureus and Klebsiella pneumonia based on bacitracin-modified Fe3O4@PDA magnetic beads combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry.

    Author: Feng Qin, Shujuan Wang, Mingxia Gao  and  Xiangmin Zhang

    Marker: RTD6110,彩虹预染超低分子量蛋白(1.2-45 kD)

    Journal: Analytical Methods. 2021,13, 2804-2811

    InstitutionDepartment of Chemistry, Fudan University

    Paper linkhttps://pubs.rsc.org/en/content/articlelanding/2021/ay/d1ay00614b

     

    8. [2021 IF=3.74] Cloning, expression, and function of ferritins in the tick Haemaphysalis flava.

    Author: Yu Zhao, Lei Liu, Jin-Bao Liu, Cong-Ying Wu, De-Yong Duan, Tian-Yin Cheng

    Marker: RTD6142 高分子量非变性电泳蛋白质Marker II(45-669 kD)

    Journal: Ticks and Tick-borne Diseases. Volume 13, Issue 2, March 2022, 101892

    InstitutionCollege of Veterinary Medicine, Hunan Agricultural University,

    Paper linkhttps://linkinghub.elsevier.com/retrieve/pii/S1877959X21002454

     

    9. [2021 IF=0.886] Purification and Characterization of an Intracellular β-Glucosidase from the Protoplast Fusant of Aspergillus oryzae and Aspergillus niger

    Author: F.M. Zhu, B. Du, H.S. Gao, C.J. Liu, and J. Lia

    Marker: RTD6109 高分子量蛋白质Marker(43-200 kD)

    Journal: Applied Biochemistry and Microbiology, 2010, Vol. 46, No. 6, pp. 626–632

    InstitutionCollege of Food Science and Technology, Hebei Normal University of Science and Technology

    Paper linkhttps://link.springer.com/article/10.1134/S0003683810060116

     

    10. [2021 IF=4.374] Effect of Fuzhuan brick-tea addition on the quality and antioxidant activity of skimmed set-type yoghurt.

    Author: Dong Liu

    Marker: RTD6103 低分子量蛋白质Marker II(14.4-116 kD)

    Journal: International Journal of Dairy Technology, Vol 71 March 2018 Pages 22-33

    InstitutionDepartment of Food Processing Technology, Anqing Vocational & Technical College

    Paper linkhttps://doi.org/10.1111/1471-0307.12395

     

    11. [2021 IF=3.7] Smart delivery of poly-peptide composite for effective cancer therapy

    Author: Xin Yuan, Yingzhou Qin, Qingmei Tian, Cuijuan Liu, Xiangzhou Meng, Bo Qie, Fan Gao, Ying Huang, Guanghui Xu, Guang Yang, Yimin Zhu

    Marker: Protein Marker

    Journal: Biomedical Materials, 2022 Jan 24;17(2)

    Institution:School of Nano-Tech and Nano-Bionics, University of Science and Technology of China

    Paper linkhttps://pubmed.ncbi.nlm.nih.gov/34996052/

     


TOP】【打印本页】【关闭窗口



中科瑞泰(北京)生物科技有限公司 版权所有 京ICP备12052607 网站管理   快递查询   IF查询
电话:010-58437227 免费电话:400-699-0631 传真:010-57909566
点击这里给我发消息   内勤订货QQ:2446698252   技术支持QQ:460449375 电子邮箱:real-times@vip.163.com
注意:公司所有产品仅供科研使用
_×
在线咨询