产品描述

Recombinant Human Matrix Metalloproteinase-1 is produced by our Mammalian expression system and the target gene encoding Phe20-Asn469 is expressed with a 6His tag at the C-terminus.The proenzyme needs to be activated by APMA for an activated form.

蛋白编号

P03956

产品别称

Interstitial Collagenase; Fibroblast Collagenase; Matrix Metalloproteinase-1; MMP-1; MMP1; CLG

分子量

52.88 KDa

表观分子量

56 KDa, reducing conditions

剂型描述

Supplied as a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, 2mM CaCl2, 1mM DTT, 0.05%Brij35, 10% Glycerol, pH 5.0.

内毒素

Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

纯度-SDS-PAGE

Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

保存条件

Store at ≤-70°C, stable for 6 months after receipt.

Store at ≤-70°C, stable for 3 months under sterile conditions after opening.

Please minimize freeze-thaw cycles.

运输条件

The product is shipped on ice packs.

Upon receipt, store it immediately at the temperature listed below.

产品背景

Matrix Metalloproteinase-1 (MMP-1) is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP1 contains several distinct domains: a prodomain that is cleaved upon activation, a catalytic domain containing the zinc binding site, a short hinge region, and a carboxyl terminal (hemopexin like) domain. MMP-1 can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, α1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL1, IGFBP3, IGFBP5, pro-MMP2, and pro-MMP9. A significant role of MMP1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into 3/4, 1/4 fragments. MMP1 may also be involved in enzyme cascades, cytokine regulation and cell surface molecule modulation.